By Arthur Cronquist

-- Natural History

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It is also feasible to use other method to extract DNA. Just make sure DNA concentration is above 1 μg/μL. Keep DNA as pure as possible. It is necessary to check DNA quality by gel electrophoresis for supercoiled form. 6. If 600 μL of water is added at one time, the quality of gold suspension is not as good, whereas repeated addition of 200 μL water followed by vortexing works better. 7. For co-bombardment, the amount of each plasmid has to be tested on different molar ratios of each construct.

Plant Mol Biol 75:621–631 Chapter 4 Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants Yuan Hu Xuan, Thomas Peterson, and Chang-deok Han Abstract Closely-located transposable elements (TEs) have been known to induce chromosomal breakage and rearrangements via alternative transposition. To study genome rearrangements in rice, an Ac/Ds system has been employed. This system comprises an immobile Ac element expressed under the control of CaMV 35S promoter, and a modified Ds element.

2. In the third round of stacking, in which a plasmid containing DsRed was added to molecule ABC−, 2 out of 20 chloramphenicol and ampicillin resistant clones (10 %) showed PCR bands amplified by primer sets i + j and a + c, consistent with the ABCD structure (Fig. 4a, b). Two anomalous clones were positive for primer sets a + c, but negative for primer sets i + j (lane X). As for the ABCD′ structure, we did not design primers to detect this configuration. 8 kb (Fig. 4 kb. As above, a representative molecule ABCD was subjected to the in vitro Cre-lox reaction prior to transformation into E.

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